Thursday, January 15, 2009

My wierd questions

Life has been blog-dry for some time now.
This tag is intended to tickle the funny bone in all you guys......I got a mail this morning that required me to answer the following questions as part of some survey( god knows what it must be for!!)So here goes........

Q: Do you believe that the cup is half empty or half full?
A: Depends on its contents…


Q: Are you an outdoor or an indoor person?
A: Depends on what you wanna do. I mean, I can’t be trekking on my sofa, right?


Q: Where do you see yourself in five years?
A: FIVE YEARS! You’ve gotta be freakin' kidding me!


Q: What are you most proud of in your life?
A: My ass..................period. :p


Q: Romance or Kinky Sex?
A: A heady mix of both!


Q: Would you rather be hot or cold?
A: Is the question implying whether I prefer being in a cold or hot place? Cold definitely, with a hot partner and a bottle of scotch.


Q: Would you rather lose an arm or a leg?
A: What kind of sadist thought of this question???


Q: Favorite element?
A: Carbon. (cuz that’s what gives us the DIAMONDS baby !!!)


Q: What was your last thought?
A: “What is my favourite element”(flash** periodic table chemistry book**)


Q: Have you had a beer in the last week?
A: I don’t drink beer. Get me tequila!


Q:Favorite body part?
A: Whose? Mine? I think I already answered that :D


Q:Do you like bananas?
A:Yes I do like to eat them. ....................*And the point was????*


Paranoia?not that i'm liking it but utterly convinced that its finally starting to show!!
WTF!!!

Thursday, January 8, 2009

Of cloned plasmids and stubborn proteins..........

My posts are getting fewer by the day,the reason? i am working my ass off in my lab.
This is specially for those computer geeks who think my work is not half as much hard work as theirs.
This is also for those who are still partying and holidaying.........the new year has long begun my friend and that too with a bang!! But for us meaner and humbler creatures of the dust its all about working on weekends..........:-(

Well for my six-month term project i am working with E.coli and Lambda phage.Its basically a study of certain protein-protein and protein-DNA interactions when an E.coli is infected by a phage.I had to initially construct plasmids which had my concerned proteins under study cloned tandem into them.

It was working as i had planned(after reading a dozen papers on the subject) and i was proud of my pace!!..........i always am at many other things as well!! Then then came my roadblock,after purifying the proteins i couldn't get them to express on the Western Blot.
Patience i thought would pull me through here but stubborn that these proteins are,i didn't get a single blot band on the membrane.To make things worse,i used a wrong reagent today and screwed it all up.

Now i am clueless as to what's my next step should be.............maybe i should prepare the primary and secondary antibodies for the blot again.
What the FUCK!!!!!!!!!! thats four days of my work,staying in that lab till 9pm gone.
Life's a BITCH!!!!

Sunday, January 4, 2009

My life's blah blah!!!!.......

Sorry, everyone…it’s finally “Me, me, me!” time again. I am once again here to take over the whole universe and spam it with only talk about myself. You don’t deserve to be treated this way-but, hey…it is the only time when I can really focus on myself so much. In the regular world, it is about everyone else. (Or is it?)

For those who wonder…. I’m fine, good even, which says a lot for me, even though I havn’t written much.It’s been hectic, and all of you have lives to so i’m sure you know how it goes.
Incredible how the days and weeks fly by, one blurred strip of color after another…A few highlights, a few issues, and my life can be placed neatly into a square little box, packed away and ready for someone else to open. Maybe a gift to someone? I don’t know really because I hardly get comments at all. It just tells me how uniquely similar I am to everyone else.

Few months back I was madly in love… Stupid, blind,dumb head-over-heels in love.
I can at least tell you that I am not with that person now, that I know. But then I ask myself ........"What does it matter, the grass is no greener elsewhere, only more freshly planted, the roots still shallow".
This time last year I knew where I would be this time, this year… I never doubted that I would still be in love.
Maybe it's time again for my barrelfull of alcohol and some serious post-coital cuddling!!

Wednesday, December 31, 2008

Of love,vodka and "shesher kobita"

I am thankful that my weekends have finally got blurry and noisy thanks to those zillion shots of vodka and midnight phone calls that seem to last effortlessly for hours.Also i am finally discovering Kolkata through my innumerable trips in the crowded city buses and the metro.It's not realy half as bad.
And it's great to be in love again...........ya i know I've said that so many times that it's become an inside joke that my friends laugh at but what the heck!!!

Three more things i'm realy looking forward to:
  • the Pink Floyd tribute at Someplace Else 10th jan
  • german classes at Max Mueller Bhavan from 17th jan
  • the Kolkata Book Fair after a long six years

Books that i read recently were few but long due,had to catch up with a little bit of culture once i'm in the city.Shesher Kobita by Tagore and Na-hanyate(La Nuit,French; Bengal Nights, English) by Maitreyi devi.

Wednesday, December 24, 2008

Initial in the dust........

By some predetermined chance
petal by newer petal
inwards and outwards unfold my incandescent life in space;
When you look at a petal closely for the first time,
it grows transparent:you look through it into the abyss
the way a rose-leaf thins out to a bloodless parchment..........
Or a flame splits up an oil-wick into separate stamens of orange against night's purple.

Everywhere i may go yet,you
snake around my body;out and in......
Forked memory darting here and there
withdrawn into some hidden lipless kiss.........
I used as well to dance,before
your absence turned the days
into acres of ice and created deserts out of my long nights.

Each harsh tropical day
the shadow of incommunicable memory
slants across the canvas of the present hour:
All that is not you
is so full of you............

Nuance
of your initial glances off the moonscape;
wake me with the warmth of jubilant green dances,
exhilarating ,melting..........
Forgotten are those nights on your roof,
They have all pushed me forward and i have numbly moved on.

Thursday, December 18, 2008

Yesterday no more..........

I finally came back to Kolkata, spent the first two weeks lazing around at home and doing my long overdue heap of good deeds like helping my kid sister with her schoolwork or helping mom around the kitchen a little bit. Then came the PG hunting, I had no idea that finding a decent PG accommodation in Salt lake would be so hectic and confusing. Anyway I finally got a good one and shifted last Sunday much to my parents’ relief (atleast it seemed so!!).

My good deeds also included my customary visit to my alma-mater to meet my old teachers. Visiting school this time was a revelation of sorts. It made me realise the fantastic world we once existed in and how we succumbed to its pseudo comfort. Those blurred nostalgic moments come flashing by everytime raw ruthless competition bangs straight on my face. The world I live in now is rudely and curtly “real”. Even now there’s a little girl somewhere inside who pops her head from nowhere and leaves me in the labyrinth of bittersweet memories.

College however taught me the lessons of a lifetime. Here memories come with cold expiry dates. I was always among the top five so no one dared to sneer at me because they needed my help. No one praised me either. My voice wasn’t mellifluous, dance never captured my toes and sports was just not for me. I was yet another obscure face in the sea of the faceless.

With just six months left for masters to get over I now know that life will never be a cakewalk anymore. It taught me to trust my enemies, they don’t flatter me. It revealed that there are more frustrations than excitements in life for people like us who are not born with a silver spoon, that success is perched above a thousand rickety stairs of failure. It made the stubborn me realise that I had 40 classmates and hardly one true friend. And then when those stipulated 5 years went by it declared it didn’t need me anymore and I subtly conveyed that I didn’t need it either.

Lastly it shouted shamelessly at me, you live one day and survive everyday.

Wednesday, December 17, 2008

Want

Want a cold cloudy day so that i can oversleep
Want to catch the bus on time
Want to be with family
Want to stop bothering about the future
Want to tell a friend how much he means to me
Want to have a good cry and not feel guilty about it later
Want to stop sulking over past relationships
Want loud noisy blurry weekends
Want to be with someone who cares
Want to sit on the roof with my 3rd bottle of vodka and fight with the stars
Want to reach out to a special someone i've been ignoring
Want to be loved.